Mechanistic Links Between Gut Dysbiosis, Insulin Resistance, and Autism Spectrum Disorder
https://url-shortener.me/83ZF Autism spectrum disorder (ASD) is a neurodevelopmental condition frequently associated with gastrointestinal symptoms, gut dysbiosis, and metabolic dysfunctions such as insulin resistance (IR). Recent evidence suggests that the gut microbiota may influence both metabolic and neurological processes through the gut–brain–metabolic axis. This review explores the molecular mechanisms linking dysbiosis, IR, and ASD, focusing on pathways such as TLR/NF-κB activation, PI3K/Akt/mTOR disruption, and the action of microbial metabolites, like short-chain fatty acids (SCFAs), lipopolysaccharide (LPS), and γ-aminobutyric acid (GABA). We discuss how dysbiosis may contribute to increased intestinal permeability, systemic inflammation, and neuroimmune activation, ultimately affecting brain development and behavior. Common microbial alterations in ASD and IR—including increased Clostridium, Desulfovibrio, and Alistipes, and reduced Bifidobacterium and butyrate-producing genera—suggest a shared pathophysiology. We also highlight potential therapeutic strategies, such as microbiota modulation, insulin-like growth factor 1 (IGF-1) treatment, and dietary interventions. Understanding these interconnected mechanisms may support the development of microbiota-targeted approaches for individuals with ASD metabolic comorbidities. https://doi.org/10.3390/ijms26136537
Experimental Characterization of a Commercial Photovoltaic Thermal (PVT) Hybrid Panel Under Variable Hydrodynamic and Thermal Conditions
https://url-shortener.me/83Z1 Photovoltaic thermal (PVT) hybrid systems offer a promising approach to maximizing solar energy utilization by combining electricity generation with thermal energy recovery. This study presents an experimental evaluation of a commercially available PVT panel, focusing on its thermal performance under varying inlet temperatures and flow rates. The work addresses a gap in the literature regarding the real-world behavior of integrated systems, particularly in residential settings where space constraints and energy efficiency are crucial. Experimental tests were conducted at three mass flow rates and five inlet water temperatures, demonstrating that lower inlet temperatures and higher flow rates consistently improve thermal efficiency. The best-performing condition was achieved at 0.012 kg/s and 10 °C. These findings deepen our understanding of the panel’s thermal behavior and confirm its suitability for practical applications. The experimental platform developed in this study also enables standardized PVT testing under controlled conditions, supporting consistent evaluation across different settings and contributing to global optimization efforts for hybrid solar technologies. https://doi.org/10.3390/en18133373
Evaluation of Mechanical Behavior and Application Potential of Totora (Schoenoplectus californicus)-and-Recycled Low-Density Polyethylene–Aluminum (LDPE–Al) Hybrid Panels
https://url-shortener.me/83YT The growing demand for sustainable materials has driven significant interest in composites reinforced with organic fibers, due to their mechanical performance, availability, and reduced environmental impact. This study investigates the mechanical behavior of two composite configurations: a cross-woven fabric and a sandwich-type panel, both made from totora (Schoenoplectus californicus) and low-density polyethylene–aluminum (LDPE–Al). Our experimental results show that the cross-woven variant achieved higher impact resistance (2.51 J), tensile strength (5.82 MPa), and greater deformation capacity (6.76%), making it more suitable for applications requiring energy absorption and flexibility, such as interior cladding and modular furniture. In contrast, the sandwich configuration exhibited superior stiffness (910 MPa), favoring structural panels and low-load roofing uses. This research distinguishes itself by integrating biodegradable totora fibers with recycled LDPE–Al to fabricate sustainable construction components, advancing circular economy principles while addressing limitations in previous composite formulations through improved mechanical balance and application-specific performance. https://doi.org/10.3390/buildings15132212
Photopolymer Flexographic Printing Plate Mold for PDMS Microfluidic Manufacture
https://url-shortener.me/83YE Flexographic printing, traditionally used in the packaging industry, has emerged as a promising technology for microfluidic device fabrication due to enabling high resolution and being commercially available at a low cost compared to conventional techniques. This review explores the adaptation of a photopolymer flexographic printing plate mold (FMold) for microfluidics, examining its advantages, challenges, and applications. It offers a state-of-the-art view of the application of FMold for microfluidic systems, which offers a unique opportunity in terms of cost-effectiveness, scalability, and rapid prototyping. Applications are diverse: FMold has enabled the fabrication of microfluidic devices used in enhanced oil recovery to prepare rock-on-a-chip models, droplet generation and storage, suspension cell culture, monoclonal antibody production, complex cell differentiation pattern creation, phage screening, drug screening, cell detection, and cancer stem cell culture. Since its first appearance in 2018, FMold has been utilized in 50 publications in different laboratories around the world. Key advancements, current research trends, and future prospects are discussed to provide a comprehensive overview of this evolving tool. https://doi.org/10.3390/polym17131723
Chrononutrition and Energy Balance: How Meal Timing and Circadian Rhythms Shape Weight Regulation and Metabolic Health
https://url-shortener.me/83Y5 Obesity and metabolic disorders remain major global health concerns, traditionally attributed to excessive caloric intake and poor diet quality. Recent studies emphasize that the timing of meals plays a crucial role in determining metabolic health. This review explores chrononutrition, a growing field that examines how food intake patterns interact with endogenous circadian rhythms to influence energy balance, glucose and lipid metabolism, and cardiometabolic risk. The circadian system, which includes a central clock in the suprachiasmatic nucleus and peripheral clocks in metabolic tissues, regulates physiological functions on a 24 h cycle. While light entrains the central clock, feeding schedules act as key synchronizers for peripheral clocks. Disrupting this alignment—common in modern lifestyles involving shift work or late-night eating—can impair hormonal rhythms, reduce insulin sensitivity, and promote adiposity. Evidence from clinical and preclinical studies suggests that early time-restricted eating, where food intake is confined to the morning or early afternoon, offers significant benefits for weight control, glycemic regulation, lipid profiles, and mitochondrial efficiency, even in the absence of caloric restriction. These effects are particularly relevant for populations vulnerable to circadian disruption, such as adolescents, older adults, and night-shift workers. In conclusion, aligning food intake with circadian biology represents a promising, low-cost, and modifiable strategy to improve metabolic outcomes. Integrating chrononutrition into clinical and public health strategies may enhance dietary adherence and treatment efficacy. Future large-scale studies are needed to define optimal eating windows, assess long-term sustainability, and establish population-specific chrononutritional guidelines. https://doi.org/10.3390/nu17132135
Biological Impact of True-to-Life PET and Titanium-Doped PET Nanoplastics on Human-Derived Monocyte (THP-1) Cells
https://url-shortener.me/83XL In the environment, plastic waste degrades into small particles known as microplastics and nanoplastics (MNPLs), depending on their size. Given the potential harmful effects associated with MNPL exposure, it is crucial to develop environmentally representative particles for hazard assessment. These so-called true-to-life MNPLs are generated through in-house degradation of real-world plastic products. In this study, we produced titanium-doped nanoplastics (NPLs) from opaque polyethylene terephthalate (PET) milk bottles, which contain titanium dioxide as a filler. The resulting PET(Ti)-NPLs were thoroughly characterized using scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), mass spectrometry (MS), dynamic light scattering (DLS), ζ-potential measurements, transmission electron microscopy (TEM), and Fourier-transform infrared (FTIR) spectroscopy. Human-derived THP-1 monocytes were employed to investigate particle uptake kinetics, dosimetry, and genotoxicity. A combination of flow cytometry and inductively coupled plasma mass spectrometry (ICP-MS) enabled the quantification of internalized particles, while the comet assay assessed DNA damage. The results revealed dose- and time-dependent effects of PET(Ti)-NPLs on THP-1 cells, particularly in terms of internalization. Titanium doping facilitated detection and influenced genotoxic outcomes. This study demonstrates the relevance of using environmentally representative nanoplastic models for evaluating human health risks and underscores the importance of further mechanistic research. https://doi.org/10.3390/nano15131040